Detection of DNA of Rickettsia and Orientia tsutsugamushi in rodents and ectoparasites in Ha Giang province

Le Thi Lan Anh, Vo Viet Cuong, Trinh Van Toan, Ho Thi Hong Nhung, Le Thi Van Anh, Can Thi Thu Thuy, Pham Thi Ha Giang, Bui Thi Thanh Nga, Bui Thi Lan Anh, Nguyen Van Chau
Author affiliations

Authors

  • Le Thi Lan Anh
  • Vo Viet Cuong
  • Trinh Van Toan
  • Ho Thi Hong Nhung
  • Le Thi Van Anh
  • Can Thi Thu Thuy
  • Pham Thi Ha Giang
  • Bui Thi Thanh Nga
  • Bui Thi Lan Anh
  • Nguyen Van Chau

DOI:

https://doi.org/10.15625/1811-4989/18/3/13892

Keywords:

mice, chigger mites, nested PCR, O. tsutsugamushi, Real-time PCR, Rickettsia spp.

Abstract

Rickettsial fever is one of a zoonotic disease which is caused by bacteria genus Rickettsia. The ectoparasites such as ticks, mites, fleas, lice... were demonstrated as the main transmited vectors through host reservoirs are rodents and small animals including mice, squirrels, mink... In this study, the rodents and ectoparasites species were identified. The molecular detection of Rickettsia was also performed. In 2018, 83 rats were trapped in 2 villages Thanh Duc and Phu Linh, Vi Xuyen district, Ha Giang province, in which 48.2% mice were found as house mice Rattus flavipectus, 21.7% was forest mice R. rattus, 12% was R. fulvescens, 8.4% was R. nitidus, the remaining rates were R. bowersi, Mus. pahari, Leopoldamys sabanus, Mus musculus and R. niviventer, accounting for 1.2% - 3.6%. The ectoparasites survey found 5 chigger mite species including Leptotrombidium (Leptotrombidium) deliense, Ascoschoengastia (Laurentella) indica, Garhliepia (Walchia) rustica, Lorilutum oreophilum and Shunsenia sp as well as 3 gamasid mite species such as Laelaps (Echidninus) sedlaceki, Laelaps (Laelaps) nuttali and Lenstivalius klossi bispiniformis. The result indicated that 19.3% and 10.8% mice were positive with Ricketsia spotted fever group (SFG) and Rickettsia typhi, respectively by real-time PCR. The nested PCR result showed that 19.4% R. flavipectus mice and 10% L. (L.) deliense chigger mites were positive with Orientia tsutsugamushi

Downloads

Download data is not yet available.

References

Blanda V, Torina A, La Russa F, D’Agostino R, Randazzo K, Scimeca S, et al. A retrospective study of the characterization of Rickettsia species in ticks collected from humans. Ticks Tick Borne Dis. 2017; Jun;8(4):610-614.

Parola P, Paddock CD, Socolovschi C, Labruna MB, Mediannikov O, Kernif T, et al. Update on tick-borne rickettsioses around the world: A geographic approach. Clinical Microbiology Reviews. 2013; 26(4):657-702.

Vu Trung N, Hoi LT, Thuong NTH, Toan TK, Huong TTK, Hoa TM, et al. Seroprevalence of scrub typhus, typhus, and spotted fever among rural and urban populations of northern Vietnam. Am J Trop Med Hyg. 2017; 96(5):1084-1087.

Hotta K, Pham HTT, Hoang HT, Trang TC, Vu TN, Ung TTH, et al. Prevalence and Phylogenetic Analysis of Orientia tsutsugamushi in Small Mammals in Hanoi, Vietnam. Vector-Borne Zoonotic Dis. 2016; 16(2):96-102.

Lê Thành Đồng, Đoàn Bình Minh, Phạm Nguyễn Thúy Vy. Xác định sự hiện diện các vi khuẩn gây bệnh trên ve, mò, mạt. Tạp chí Y học dự phòng. 2017;157–65.

Nguyễn Văn Minh, Nguyễn Văn Tình và cs. Đặc điểm di truyền phân tử của vi khuẩn Orientia tsutsugamushi gây bệnh sốt mò ở một số tỉnh phía bắc. Tạp chí KH&CN nhiệt đới. 2017; 13(11):59-66.

Tzianabos T, Anderson BE, McDade JE. Detection of Rickettsia rickettsii DNA in clinical specimens by using polymerase chain reaction technology. Journal of Clinical Microbiology. 1989; Dec;27(12):2866-8.

Phan JN, Lu CR, Bender WG, Smoak RM, Zhong J. Molecular Detection and Identification of Rickettsia Species in Ixodes pacificus in California. Vector-Borne Zoonotic Dis. 2011; Jul; 11(7): 957–961.

Do Amaral RB, Lourenço EC, Famadas KM, Garcia AB, Machado RZ, André MR. Molecular detection of Bartonella spp. and Rickettsia spp. in bat ectoparasites in Brazil. PLoS One. 2018; 13(6): e0198629.

Bartlett JG. Rickettsia parkeri: A newly recognized cause of spotted fever rickettsiosis in the united states. Infect Dis Clin Pract. 2004; Mar 15;38(6):805-11.

Prakash J a J, Abraham OC, Mathai E. Evaluation of tests for serological diagnosis of scrub typhus. Trop Doct [Internet]. 2006; 36(4):212–3.

Kamani J, Baneth G, Apanaskevich DA, Mumcuoglu KY, Harrus S. Molecular detection of Rickettsia aeschlimannii in Hyalomma spp. ticks from camels (Camelus dromedarius) in Nigeria, West Africa. Med Vet Entomol. 2015; Jun;29(2):205-9.

Labruna MB, McBride JW, Bouyer DH, Camargo LMA, Camargo EP, Walker DH. Molecular Evidence for a Spotted Fever Group Rickettsia Species in the Tick Amblyomma longirostre in Brazil. J Med Entomol. 2009; May;41(3):533-7.

Ishikura M, Ando S, Shinagawa Y, Matsuura K, Hasegawa S, Nakayama T, et al. Phylogenetic analysis of spotted fever group rickettsiae based on gltA, 17-kDa, and rOmpA genes amplified by nested PCR from ticks in Japan. Microbiol Immunol. 2003.

Prakash JAJ, Sohan Lal T, Rosemol V, Verghese VP, Pulimood SA, Reller M, et al. Molecular detection and analysis of spotted fever group Rickettsia in patients with fever and rash at a tertiary care centre in Tamil Nadu, India. Pathog Glob Health. 2012; Mar;106(1):40-5.

Đặng Huy Huỳnh, Cao Văn Sung, Lê Xuân Cảnh, Phạm Trọng Ảnh, Lê Xuân Đặng, Hoàng Minh Khiên, Nguyễn Minh Tâm. Động vật chí Việt Nam - Fauna of Vietnam, 25. Lớp thú (Mammalia: Primates, Carnivora, Artiodactyla, Perissodactyla, Rodentia). Nhà xuất bản Khoa học và Kỹ thuật, Hà Nội, 2008.

Nguyễn Văn Châu, Đỗ Sĩ Hiển, Nguyễn Thu Vân, 2007. Động vật chí Việt Nam – Fauna of Vietnam, 16. Họ mò đỏ Trombiculidae, Bộ bọ chét Siphonaptera. Nhà xuát bản Khoa học và Kỹ thuật: 306 trang.

Nguyễn Văn Châu, Trần Thanh Dương. Tài liệu định loại Ve (Ixodida: Ixodoidea), Mò (Prostigmata: Trombiculidae), Mạt (Mesostigmata: Gamasoidea) thường gặp ở Việt Nam. Nhà xuất bản Y học, Hà Nội, 2016.

Nguyễn Văn Châu, Nguyễn Mạnh Hùng, Hồ Đình Trung. Thực hành kỹ thuật chân đốt y học. Nhà xuất bản Y học, Hà Nội, 2011.

S. P. Frances, P. Watcharapichat, D. Phulsuksombati and P. Tanskul. Transmission of Orientia tsutsugamushi, the aetiological agent for scrub typhus, to co-feeding mites. Parasitology (2000), 120, 601–607.

Leulmi H, Aouadi A, Bitam I, Bessas A, Benakhla A, Raoult D, et al. Detection of Bartonella tamiae, Coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern Algeria. Parasites and Vectors. 2016; Jan 20;9:27.

Cortez V, Canal E, Dupont-Turkowsky JC, Quevedo T, Albujar C, Chang TC, et al. Identification of Leptospira and Bartonella among rodents collected across a habitat disturbance gradient along the Inter- Oceanic Highway in the southern Amazon Basin of Peru. PLoS One. 2018; 13(10):e0205068.

Downloads

Published

28-11-2020

How to Cite

Lan Anh, L. T., Viet Cuong, V., Van Toan, T., Thi Hong Nhung, H., Van Anh, L. T., Thi Thu Thuy, C., Thi Ha Giang, P., Thi Thanh Nga, B., Thi Lan Anh, B., & Van Chau, N. (2020). Detection of DNA of Rickettsia and Orientia tsutsugamushi in rodents and ectoparasites in Ha Giang province. Vietnam Journal of Biotechnology, 18(3), 543–552. https://doi.org/10.15625/1811-4989/18/3/13892

Issue

Section

Articles

Most read articles by the same author(s)

1 2 > >>