EXTRACTION AND PURIFICATION OF UNSATURATED FATTY ACIDS FROM HETEROTROPHIC MARINE MICROALGAE SCHIZOCHYTRIUM MANGROVEI PQ6 BIOMASS

Hoàng Thị Lan Anh
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Authors

  • Hoàng Thị Lan Anh Viện công nghệ sinh học

DOI:

https://doi.org/10.15625/1811-4989/7/3/12455

Keywords:

DHA, DPA, long-chain polyunsaturated fatty acid, Schizochytrium mangrovei PQ6

Abstract

Using a simple and inexpensive procedure involving methylation in dried biomass, urea complexation and

winterization in a sequential way, we succeeded in exfracting and purificating of unsaturated fatty acid,

especially docosahexaenoic acid (DHA, C22:6n-3) and docosapentaenoic acid (DPA, C22:5n-6) from

Schizochytrium mangrovei PQ6 biomass isolated in Phu Quoc island, Kien Giang province on 2006. With the urea/fatty acid ratio was 5:2 at crystallization temperature from 4 to 10°C for 12 hours, total fatty acid was divided into two fractions: non-urea complexing (liquid phase) and urea complexing fraction (solid phase). The non-urea complexing fraction contained a highest amount of DHA and DPA (85.2% and 13.4% total fatty acid  of liquid phase, respectively). In the confrast, urea complexing fraction contained mainly saturated fatty acid including palmitic acid more than 55.9% of total fatty acid in this phase. For the reason, this procedure was appropriated for DHA, DPA exfraction and purification from S. mangrovei PQ6 biomas

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Published

22-11-2011

How to Cite

Anh, H. T. L. (2011). EXTRACTION AND PURIFICATION OF UNSATURATED FATTY ACIDS FROM HETEROTROPHIC MARINE MICROALGAE SCHIZOCHYTRIUM MANGROVEI PQ6 BIOMASS. Vietnam Journal of Biotechnology, 7(3), 381–387. https://doi.org/10.15625/1811-4989/7/3/12455

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