Selection of fermentation condition for expression of recombinant single chain antibody recognizing the antigen of blood type a in Escherichia coli

Dang Thi Ngoc Ha, Le Thi Thu Hong, Do Thi Huyen, Truong Nam Hai

Abstract


Single chain recombinant antibody identifying blood group A antigen in the ABO system (antiA_scFv) was constructed to express in E. coli. In this study, we optimized fermentation conditions to increase the yield of antiA_scFv expression as well as to reduce cost and save culture time. The results showed that the antiA_scFv expression was highest in the culture TB medium with 0.1 mM IPTG, at 30oC. Induction time point was in exponential growth phase of cell with cell density OD600 = 1.5. With the selected fermentation conditions, the cell biomass increased 5.2 times (from OD600 = 3.27 to OD600 = 17.02) and antiA_scFv yield increased significantly as compared to the initial conditions.


Keywords


Escherichia coli, kháng thể đơn chuỗi tái tổ hợp, kháng nguyên nhóm máu A

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Published by Vietnam Academy of Science and Technology