Disease-free plant production and rapid propagation of some Cymbidium cultivars by meristem culture

Phan Xuan Huyen, Nguyen Trung Ai, Nguyen Thi Lang, Nguyen Thi Dieu Huong, Dinh Van Khiem, Duong Tan Nhut

Abstract


In this paper, the protocol for disease-free plant production and rapid propagation of some Cymbidium cultivars by meristem culture are presented.

Proliferation and multiplication rate of PLB were promoted in MS medium supplemented with 0.2 mg/l NAA and 2.0 mg/l BA.

To increase the multiplication rate, PLB was cut in to 4 parts for subculture. This cutting way was better as compared to that of 2 parts.

Shoots were well rooted on MS medium plus 0.5 mg/l NAA.

100% survival rate of Cymbidium plantlets was obtained after transferring to the greenhouse.

This protocol can be applied for the micropropagation of many Cymbidium cultivars with great number and uniform plantlets.


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DOI: https://doi.org/10.15625/0866-7160/v26n1.6868 Display counter: Abstract : 28 views. PDF : 35 views.

 

                 

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