Enhancing expression of recombinant fimbrial adhesin K88 isolated of enterotoxigenic Escherichia coli from piglet

Nguyen Hoang Loc, Nguyen Thi Khanh Quynh, Hoang Tan Quang, Tran Thuy Lan, Le My Tieu Ngoc, Dinh Thi Bich Lan, Phung Thang Long

Abstract


Fimbrial adhesins are important toxic factors of enterotoxigenic E. coli (ETEC) strains, whereby they have the ability to adhere to small intestine of the host. K88 (F4) is the first fimbrial adhesin was detected in ETEC strains from piglet, and it is the most popular fimbrial adhesin of E. coli strains causes diarrhea in weaned piglets. In previous report, we successful expressed the faeG gene encoding fimbrial adhesin K88-1NT in recombinant E. coli BL21 (DE3). Highest level of K88-1NT was determined after induction by 0.1% lactose for 6 h at 35oC when cell density (OD600) reached a value of 0.5. Induction medium including Na2HPO4.12H2O 1%, KH2PO4 0.3%, NaCl 0.05%, (NH4)2SO4 1%, MgSO4.7H2O 0.2%, glucose 1.5%, tryptone 1% and yeast extract 1%. Purified K88-1NT protein from Ni2+ affinity chromatography had a molecular weight of 31 kDa.


Keywords


Escherichia coli, enterotoxin, fimbrial adhesin, weaned piglet.

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DOI: https://doi.org/10.15625/0866-7160/v36n1se.4378

Published by Vietnam Academy of Science and Technology