Evaluation of porcine reproductive and respiratory syndrome virus (PRRSV) in Binh Duong province by Elisa and RT-PCR

Truong Thi Diem Hang, Nguyen Ngoc Hai

Abstract


Porcine reproductive and respiratory syndrome (PRRS), which cause heavy losses to the breeding economy in many countries, including Vietnam, has been a top concern of the farm. Testing PRRSV antibodies and the presence of PRRSV have an important role in the evaluation and control of PRRS. The method used to diagnose PRRS are ELISA and RT-PCR, however, these methods are often used individually, not combined, lead to errors in judgment PRRS status of the herd. In this study, ELISA, RT-PCR and PCR steps 2 are combined, to assess the status of the herd PRRS circulation both antigens and antibodies. As a result, ELISA (+) - RT-PCR (-) accounted for the highest proportion of 48% (36/75). Combination ELISA
(-) - RT-PCR (+) accounted for 5.333% (4/75) and this consortium focused in farm 3. Combination ELISA (+) - RT-PCR (+) accounted for 4% (3/75). In a total of 75 pigs, there were 42.67% negative for both reactions. The pig was the conclusion is negative for PRRSV and retained.


Keywords


Porcine reproductive and respiratory syndrome, virus desease, RT-PCR.

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DOI: https://doi.org/10.15625/0866-7160/v36n1se.4336

Published by Vietnam Academy of Science and Technology