Biological characteristics of bacterial strains degraded 1,2-Dicloroethane isolated in Vietnam

Pham Thi Vui, Pham Thi Hoa, Pham Bao Yen, Nguyen Quang Huy


The use of pesticides has become an integral part of modem agricultural techniques, but increased use of these chemicals might cause the environmental pollution with impact on bats ecosystem and human health. 1,2-dicholoroethane (1,2-DCE) is one toxic of the chemicals commonly used for plant protection that may cause the cancer and environment pollution.

In nature, microorganisms are regarded as active factor in transformation cycle of chemical elements and balance environment. Adaptation of indigenous soil microbial populations occurs as a result of contact with xenobiotics. From the soil and water samples collected in the area that use plant protection products in Hanoi, 45 strains were isolated in culture added 1,2-DCE as carbon source. Two bacterial strains R9 and S15 are more active than others. Based on morphological, biological characteristics and sequences of 16S rDNA, the two isolated strains were classified of Pseudomonas and Klebsiella. The R9 and S15 strains are similar to Pseudomonas and Klebsiella at 99.9% and 99.5% respectively. Both strains R9 and S15 were well grown at 23°C-37oC and a concentration of 1.2 DCE from 15 to 20 mM.


Contamination, 1,2-dicholoroethane, decomposition, environment pollution, isolation.

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