Streptomyces chartreusis CP23X9 producing xylanase: biological characteristics and classification

Vu Van Loi, Nguyen Van Hieu, Nguyen Thi Hong Lien, Pham Thi Bich Hop, Pham Thi Hong Thao

Abstract


Xylan is the major component of hemicellulose that makes up for plant cell. It is constructed by 1,4-β-linked-D-xylopyranosyl units with several side-groups. Xylan can be degraded by xylanase from microorganisms such as fungi, bacteria and actinomyces. Enzyme xylanase is widely applied in industry, such as textile, paper and pulp, and food production. In this study, an Actinomyces strain CP23X9 producing xylanase was characterized and identified. The results showed that strain CP23X9 can grow well on different media with a wide range of temperature between 15-45oC; pH 5-10, and salinity of 0-6%. On the agar plate, the strain has blue aerial mycelia and pale yellow vegetative mycelia. The mature spore chains appeared spirals, moderately long, bearing 10 to 50 spores each; the spore surface spiny. The strain utilized carbon sources such as D-glucose, D-xylose, D-mannitol, L-arabinose và L-rhamnose. Strain CP23X9 capable of extracellular enzymes producing, such as ligninase, protease, amylase and with high xylanase activity (42.7 U/ml). Based on the biological characteristics and phylogenetic analysis of 16S rDNA, it can be concluded that strain CP23X9 is close to Streptomyces chartreusis (99%), hence identified as Streptomyces chartreusis CP23X9. The xylanase activity of Streptomyces chartreusis CP23X9 will be used in improved bleaching abilities in future


Keywords


Streptomyces chartreusis, 16S rDNA, Actinomyces classification, biological characteristics, xylanase.

Full Text:

PDF


DOI: https://doi.org/10.15625/0866-7160/v35n3se.3838 Display counter: Abstract : 43 views. PDF : 248 views.

 

                 

Editorial Office:

1st Floor, A16 Building, 18B Hoang Quoc Viet Street, Cau Giay District, Hanoi, Vietnam

Tel: (+84) 24 3791 7101

Email: tapchisinhhoc@vjs.ac.vn