First record of Xiphinema hunaniense Wang & Wu, 1992 (Dorylaimida: Longidoridae) associated with tea (Camellia sinensis (L.) Kuntze) in Thanh Hoa, Vietnam

Le Thi Mai Linh, Nguyen Huu Tien, Nguyen Thi Duyen, Trinh Quang Phap

Abstract


The dagger nematodes, Xiphinema spp., are migratory root-ectoparasitic nematodes that cause damage to a wide range of wild and cultivated plants over the world. In Vietnam, this nematode group has been studied mainly based on morphological characterizations. During a survey of pathogens associated with tea, a plant with many medicinal and therapeutic potentials, a population of Xiphinema hunaniense was recorded. This study provides the first morphological and molecular characterizations of Xiphinema hunaniense found on Tea in Vietnam. The 28S rDNA, and 18S rDNA phylogenetic trees of the genus Xiphinema are also provided. 18S rDNA sequence of X. hunaniense is also submitted to GenBank for the first time.

 


Keywords


18S rDNA, 28S rDNA, molecular, phylogeny, taxonomy.

References


De Ley, P., Felix, M. A., Frisse, L., Nadler, S., Sternberg, P. & Thomas, W. K. 1999. Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1: 591-612.

Gutiérrez-Gutiérrez, C., Cantalapiedra-Navarrete, C., Decraemer, W., Vovlas, N., Prior, T., Palomares-Rius, J. & Castillo, P. 2012. Phylogeny, diversity, and species delimitation in some species of the Xiphinema americanum-group complex (Nematoda: Longidoridae), as inferred from nuclear and mitochondrial DNA sequences and morphology. European Journal of Plant Pathology 134: 561-597.

Mamun, M., Ahmed, M. & Paul, S. 2011. Control of plant parasitic nematodes of tea soil using different species of green crops in Bangladesh. Tea Journal of Bangladesh 40: 1-7.

Nguyen, H. T., Le, T. M. L., Nguyen, T. D., Liebanas, G., Nguyen, T. a. D. & Trinh, Q. P. 2019. Description of Geocenamus vietnamensis sp. n. (Nematoda: Merliniidae) from Vietnam. Journal of nematology 51: e2019-25.

Nguyen, H. T., Nguyen, T. D., Le, T. M. L. & Trinh, Q. P. 2020. First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda: Longidoridae) in Vietnam. Journal of nematology 52.

Nguyễn, N. C. 2003. Tuyến trùng thực vật và cơ sở phòng trừ. NXB Khoa học Kỹ thuật Hà Nội.

Nguyen, N. C. & Nguyen, V. T. 2000. Fauna of Vietnam, Plant-parasitic nematodes. Science and Technics publishing house, Hanoi, Vietnam.

Orlando, V., Chitambar, J. J., Dong, K., Chizhov, V. N., Mollov, D., Bert, W. & Subbotin, S. A. 2016. Molecular and morphological characterisation of Xiphinema americanum-group species (Nematoda: Dorylaimida) from California, USA, and other regions, and co-evolution of bacteria from the genus Candidatus Xiphinematobacter with nematodes. Nematology 18: 1015-1043.

Perry, R. & Moens, M. 2013. Plant nematology. CABI, London, UK.

Sikora, R. A., Coyne, D., Hallmann, J. & Timper, P. 2018. Plant-parasitic nematodes in subtropical and tropical agriculture. Cabi, UK.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution 30: 2725-2729.

Wang, S. & Wu, X. 1992. Two species of Xiphinema Cobb, 1913 (Dorylaimida: Longidoridae) from around grape roots in China. Acta Phytopathologica Sinica 22: 117-123.

Wang, X., Bosselut, N., Castagnone, C., Voisin, R., Abad, P. & Esmenjaud, D. 2003. Multiplex polymerase chain reaction identification of single individuals of the longidorid nematodes Xiphinema index, X. diversicaudatum, X. vuittenezi, and X. italiae using specific primers from ribosomal genes. Phytopathology 93: 160-166.

Wu, Y., Zheng, J. & Robbins, R. T. 2007. Molecular characterization of a Xiphinema hunaniense population with morphometric data of all four juvenile stages. Journal of Nematology 39: 37-42.




DOI: https://doi.org/10.15625/2615-9023/15616 Display counter: Abstract : 119 views.

 

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