Transcription expression of gene encoding cathelicidin CATHL4 in Vietnam indigenous yellow cattle

Do Thi Tuoi, Tran Thi Thuy Anh, Pham Doan Lan, Nguyen Thi Hong Van
Author affiliations

Authors

  • Do Thi Tuoi
  • Tran Thi Thuy Anh
  • Pham Doan Lan
  • Nguyen Thi Hong Van

DOI:

https://doi.org/10.15625/2615-9023/15457

Keywords:

Vietnamese Yellow cattle, cathelicidins, CATHL4 gene, transcription level, qRT-PCR.

Abstract

Cathelicidins, a family of host-defence peptides, are present in a diverse range of species, including fish, amphibians, birds, reptiles, and mammals. The evidence for the role of these cationic antimicrobial peptides in innate host defenses is convincing from the data of animal model and transgenic animal experiments as well as wildlife or domestic animals, indicating that the peptides protect against inflammatory and immune system after induction by bacterial infection. In this study, we present the assessment of transcription expression of CATHL4 gene which encodes indolicidin, a cathelicidin from indigenous yellow cattle of Vietnam. The research focused on RNA samples extracted from lung tissues and lymph node collected from diseased cattle which died of infectious respiratory symptoms and the healthy cattle which were slaughtered for food purposes. By quantitative real-time PCR, the relative expression of transcripts was determined and analyzed using the expression of YWHAZ gene as reference. The results showed that this gene was abundantly expressed at a higher level in tissues of the diseased cattle than in those of the healthy ones. In both infection and healthy states, the expression of CATHL4 in lymph nodes were higher than in lung tissues. This indicated that CATHL4 (indolicidin) may participate in functions against infectious pathogens.

 

 

 

 

 

 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Baumann A., Kiener M. S., Haigh B., Perreten V. Summerfield A., 2017. Differential ability of Bovine antimicrobial cathelicidins to Mediate nucleic acid sensing by epithelial cells. Front. Immunol. 8(59). https://doi.org/10.3389/fimmu.2017.00059 https://doi.org/10.3389/fimmu.2017.00059">

Brahma B., Patra M. C., Karri S., Chopra M., Mishra P., Chandra De B., Kumar S., Mahanty S., Thakur K., Poluri K. M., Datta T. K., De S., 2015. Diversity, Antimicrobial Action and Structure A ctivity Relationship of Buffalo Cathelicidins. Plos One. https://doi.org/ 10.1371/journal.pone.0144741 https://doi.org/ 10.1371/journal.pone.0144741">

Broekman D. C., Frei D. M., Gylfason G. A., Steinarsson A., Jörnvall H., Agerberth B., Gudmundsson G. H., Maier V. H., 2011. Cod cathelicidin: Isolation of the mature peptide, cleavage site characterization and developmental expression. Developmental and Comparative Immunology, 35: 296–303.

Gillenwaters E. N., Seabury C. M., Elliott J. S., Womack J. E., 2009. Sequence analysis and polymorphism discovery in 4 members of the bovine cathelicidin gene family. J Hered, https://doi.org/ 101093/jhered/esn112 https://doi.org/ 101093/jhered/esn112">

Goossens K., Poucke M. V., Soom A. V., Vandesompele J., Zeveren A. V. and Peelman L. J,, 2005. Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC developmental biology 5, 27, https://doi.org/10.1186/1471-213X-5-27 https://doi.org/10.1186/1471-213X-5-27">

Livak K. J. 1997 (updated 2001). Relative quantification of gene expression: ABI Prism 7700 Sequence Detection System, Applied Biosystems User Bulletin #2.

Livak K. J., Schmittgen T. D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods, 25: 402–408.

Maier V.H., Dorn, K.V., Gudmundsdottir, B.K., Gudmundsson, G.H., 2008. Characterisation of cathelicidin gene family members in divergent fish species. Mol.Immunol. 45, 3723–3730

Shinnar A. E., Butler K. L., and Park H. J., 2003, Cathelicidin family of antimicrobial peptides: proteolytic processing and protease resistance. Bioorganic Chemistry 31, 425–436.

Zhang L., Jie H., Xiao Y., Zhou C., Lyu W. and Bai W., 2019. Genomic Identification and Expression Analysis of the Cathelicidin Gene Family of the Forest Musk Deer. Animals, 9, 481; https://doi.org/10.3390/ani9080481 https://doi.org/10.3390/ani9080481">

Mookherjee N., Brown K. L., Bowdish D. M., Doria S., Falsafi R., Hokamp K., Roche F. M., Mu R., Doho G. H., Pistolic J., Powers J. P., Bryan J., Brinkman F. S., Hancock R. E., 2006. Modulation of the TLR-mediated inflammatory response by the endogenous human host defense peptide LL-37. J Immunol, 176: 2455–2464.

Tomasinsig L., Zanetti M., 2005. The cathelicidins - structure function and evolution. Curr Protein Pept Sci.; 6: 23–34. PMID: 15638766.

Whelehan C. J, Barry R. A., Meade K. G, Eckersall P. D., Chapwanya A., Narciandi F., Lloyd A. T. and O’Farrelly C., 2014. Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue. BMC Genomics, 15: 128

Wuerth K., Hancock R. E., 2011. New insights into cathelicidin modulation of adaptive immunity. Eur J Immunol, 41: 2817–2819.

Downloads

Published

31-03-2021

How to Cite

Tuoi, D. T., Anh, T. T. T., Lan, P. D., & Van, N. T. H. (2021). Transcription expression of gene encoding cathelicidin <i> CATHL4</i> in Vietnam indigenous yellow cattle. Academia Journal of Biology, 43(1). https://doi.org/10.15625/2615-9023/15457

Issue

Section

Articles