Isolation and characterization of a c-repeat binding factor gene from Tevang-1 maize cultivar

Nguyen Thuy Linh, Luu Han Ly, Nguyen Thuy Duong, Huynh Thi Thu Hue

Abstract


C-repeat binding factor (CBF) proteins are transcription factors involved in plant response to abiotic stresses, especially low-temperature condition. In this research, a CBF3-coding gene was isolated from a cold-acclimation maize variety, Zea mays var. Tevang-1 and denoted as ZmCBF3tv. The isolated gene shared 96.49% homology with the B73-reference gene and had no intron in the coding sequence. By using bioinformatic tools, a number of variations in the nucleotide and amino acid sequences were identified. An alignment between ZmCBF3tv and other CBF/DREB1 proteins from various species revealed functional regions and typical features, such as nuclear localization signal (NLS), the AP2 DNA-binding domain, and acidic-amino-acid-rich segments. Additionally, a phylogenetic analysis based on the AP2 domain showed that the maize CBF3 transcription factor had the highest similarity with that from rice and closely related to other DREB1/CBF protein of monocots. The function of the ZmCBF3tv product is suggested to be a CBF/DREB1 transcription factor.

 

 

 

 

 


Keywords


CBF/DREB1, transcription factor, Tevang-1, maize, cold tolerance.

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DOI: https://doi.org/10.15625/0866-7160/v41n3.13782 Display counter: Abstract : 140 views. PDF : 36 views.

 

                 

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