CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OILS FROM STEMS AND LEAVES OF Michelia alba D.C. GROWING IN VIETNAM

Chu Thi Thu Ha, Tran Huy Thai, Le Ngoc Diep, Trinh Xuan Thanh, Dinh Thi Thu Thuy, Nguyen Thanh Tra, Nguyen Thi Thu Ha

Abstract


The chemical composition of the essential oils obtained by hydrodistillation of the stems and leaves of Michelia alba D.C. growing wild in North Vietnam was analyzed by GC/MS. In total, 28 compounds were characterized in both stem and leaf oils, accounting for 99.33% and 99.54% of the oils, respectively. Linalool was the major component of both oils (80.65% and 72.89%, respectively), and the same presence with various amounts of other 22 compounds constituents except 1,10-di-epi-cubenol in stem oil; and borneol (=endo-borneol), α-copaene, α-selinene and ethyl 2-phenylhexanoate in leaf oil. In the stem oil, (Z)-β-ocimene 1.94%; (E)-β-ocimene 2.28%; β-caryophyllene 1.26%; (E)-nerolidol 1.15%; caryophyllene oxide 2.26% and epi-α-muurolol (=T-muurolol) 1.80% were present in sizeable amount. The most abundant minor components of the leaf oil were (Z)-β-ocimene 1.73%; (E)-β-ocimene 2.28%; Cis- β-elemene 3.03%; β-caryophyllene 4.04%; α-humulene 1.44%; (E)-nerolidol 4.42% and caryophyllene oxide 1.36%. The essential oil samples were tested against a Gram positive bacteria strain Staphylococcus aureus, a Gram negative bacteria strain Escherichia coli, and a yeast strain Candida albican using the agar disk diffusion method. The antimicrobial activity of leaf oil was stronger than that of stem oil, and both of oils exhibited a significant inhibitory activity against all three test microorganism strains with the inhibitory zone diameters from 15 to 46 mm.

Keywords


Magnoliaceae, Michelia alba, essential oil composition, stem oil, leaf oil, antimicrobial activity

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DOI: https://doi.org/10.15625/2615-9023/v40n3.13151

Published by Vietnam Academy of Science and Technology