OPTIMIZATION OF THE ENZYME ASSISTED EXTRACTION OF ESSENTIAL OIL FROM THE LEAVES AND BRANCHES OF CINNAMOMUM CASSIA USING BOX-WILSON METHOD

Hoang Thi Bich, Le Mai Huong, Nguyen Quyet Chien, Dinh Thi Thu Thuy, Le Tat Thanh, Do Trung Sy, Le Thi Thuy Hang, Pham Hong Hai

Abstract


The process of enzyme assisted extraction of essential oil from the leaves and branches of the Vietnamese aromatic plant Cinnamomum cassia was studied and optimized using a Box-Wilson central composite design consisting of 05 independent variables (pH, temperature T, time τ, concentration of the enzyme Laccase, and concentration of the enzyme Cellic Htec2) and two dependent variables (reducing sugar and yield of essential oil). Second-order polynomial equations were obtained for the responses, which fitted well with the experimental data. Optimal conditions for oil yield were found at pH = 5.2; T = 440C; τ = 5h30'; Laccase = 0.42 ml/g, and Cellic Htec2 = 1.15%. The experimental value (0.982% oil yield) was close to the predicted value (0.978%). The application of enzyme assisted extraction  in combination with optimization using response surface methodology substantially improved the oil yield as compared with traditional method.

 


Keywords


optimization, Box-Wilson central composite design, enzyme assisted extraction, essential oil, Cinnamomum cassia

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Published by Vietnam Academy of Science and Technology