STUDY THE ANTICANCER MECHANISM OF THE PROMISSING COMPOUND 2B2D BY USING MICROARRAY TECHNIQUE

Do Thi Thao, Do Thi Phuong, Nguyen Thi Trang, Nguyen Thi Nga, Huang Ying Chi

Abstract


Being a modern technique with the ability of studying, discovering, probing and analyzing the  expression  of  thousands  genes,  even  the  whole  genome  in  the  only  one  experiment, microarray  proved  to  be  a  powerful  tool  for  cancer  research  especially  at  molecular  level. Employing  the  potential  anticancer  compound  1-(5,7-dimetoxy-2,2-dimetyl-2H-cromen-8-yl)-but-2-en-1-on  (2B2D  in  short) and LU-1,  the human  lung cancer cells as  research objects, we successfully  hybridized  the  cy3/5  incorporated  cDNA  with  Phalanx  HOV5  microarray.  The results  showed  742  genes  that  got  effected with  equal  or  over  two  folds  change  under  2B2D treatment.  Among  -  those,  386  genes  were  up-regulated  while  the  other  356  were  down-regulated. The Nuclear  factor  (erythroid-derived  2,  regulatory  factor X  domain  containing  1, fibroblast  growth  factor  receptor  3  (achondroplasia,  thanatophoric  dwarfism)  and  E2F transcription factor 8 genes were the most stimulated by our compound. The genes that named as Solute carrier  family 7  (cationic amino acid  transporter,  system) member 11,  kelch-like 24 and Hypothetical LOC344887 were the most down in action.

Keywords


cDNA, cyanine, LU-1, Mallotus apelta, microarray, spectrophotometer



DOI: https://doi.org/10.15625/0866-708X/50/3/9496 Display counter: Abstract : 13 views. PDF (Tiếng Việt) : 23 views. PDF (Tiếng Việt) : 13 views.

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Published by Vietnam Academy of Science and Technology