Study on mixing anti-A monoclonal antibody and anti-B monoclonal antibody to make anti-AB save as abo blood grouping reagent

Nguyen Thi Trung, Truong Nam Hai
Author affiliations

Authors

  • Nguyen Thi Trung Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Truong Nam Hai Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/56/1/9372

Keywords:

ABO blood group system, ABO blood grouping reagent

Abstract

So, it needs to balance the ratio of anti-A monoclonal antibody and anti-B monoclonal antibody  in the mixing so that the possibility of agglutination is the best. In this paper, anti-A monoclonal antibody (titer is 1/256) and anti-B monoclonal antibody (titer is 1/256) was used. The best results were obtained at one volume anti A monoclonal antibody is mixed one volume anti-B monoclonal antibody. The anti-A,B antibody titer was 1/128 for red blood group A and it was 1/128 for red blood group B. The intensity of agglutination reached  3+ for both red blood group A and B.

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Published

30-01-2018

How to Cite

[1]
N. T. Trung and T. N. Hai, “Study on mixing anti-A monoclonal antibody and anti-B monoclonal antibody to make anti-AB save as abo blood grouping reagent”, Vietnam J. Sci. Technol., vol. 56, no. 1, pp. 17–23, Jan. 2018.

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Section

Natural Products