Different fructose feeding strategies for poly(3-hydroxybutyrate) production by Yangia sp. ND199

Tran Huu Phong, Dang Minh Khuong, Duong Van Hop, Doan Van Thuoc
Author affiliations

Authors

  • Tran Huu Phong Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam
  • Dang Minh Khuong Bao Yen 2 High School, Bao Yen district, Lao Cai, Vietnam
  • Duong Van Hop Institute of Microbiology and Biotechnology, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam
  • Doan Van Thuoc Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/0866-708X/55/2/8677

Keywords:

Yangia sp. ND199, fed batch fermentation, PHB production, fructose

Abstract

Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highest CDW of 78.5 g/l, PHB content of 67.5 wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fed-batch fermentation, is among the highest reported so far for PHB production by halophilic bacteria.         

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Published

17-04-2017

How to Cite

[1]
T. Huu Phong, D. Minh Khuong, D. V. Hop, and D. V. Thuoc, “Different fructose feeding strategies for poly(3-hydroxybutyrate) production by Yangia sp. ND199”, Vietnam J. Sci. Technol., vol. 55, no. 2, pp. 195–201, Apr. 2017.

Issue

Section

Natural Products