QUANTITATIVE DETERMINATION OF APIGENIN FROM APIUM GRAVEOLENS L. BY HPLC-PDA METHOD

Vi Nguyen Tuong Le, Dung Tien Le, Khoa Cuu Nguyen, Tuan Ngoc Nguyen, Hung Tong Quach, Phuong Thi Nguyen, Hang Thi Le Dang, Chinh Van Nguyen, Quynh Tran Thuy Nguyen

Abstract


In recent years, the study of the structure and biological activity of medicinal plants have a particularly important to search for anti-cancer medicine. Apium graveolens L. is a species that has been studied for its ability to anti-cancer, lower blood pressure, antioxidant ... In which, apigenin is the compound that is most interested in this species. Apigenin was isolated from the ethyl acetate extract and performed column chromatography with solvent system n-hexane: ethyl acetate (from 100% to 1: 1) obtained 7 fractions. Fraction 7 was applied on column chromatography with n-hexane: ethyl acetate (2:1). The qualitative and quantitative process of apigenin in Apium graveolens L. by HPLC - PDA method was investigated using reverse phase column VDSpher PUR 100 C18 (25 cm × 4,6 mm, 5 mm). with detection wavelength at 335 nm, the mobile phase is 0.1% acetic acid: acetonitrile (60:40) with isocratic elution, flow rate 1 mL/min; injection volume 20 µL; column temperature 25℃. Validation results showed that the process had high specificity, linearity, repeatability with RSD = 1,000%, accuracy recovery with 99,97%, LOD was 0,1 μg/mL and the LOQ was 0,3 μg/mL.



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