Pham Duc Thinh


Sea cucumber glycosaminoglycans have been well known as potential anticoagulant and antithrombin agents. In this investigation, glycosaminoglycans were isolated from sea cucumber Stichopus horrens by papain enzymatic digestion. Crude glycosaminoglycans were fractionated and purified by using anion-exchange chromatography on the DEAE-Macro Prep column to give two fractions of fucosylated chondroitin sulfate (FCS1) and fucan sulfate (FS2). Structural characteristics of F1 and F2 fractions were elucidated using chemical and IR, NMR spectroscopic methods. The results showed that the monosaccharide compositions of FCS1 consist of N-Acetyl-Galactosamine (GlcNAc), D-Glucuronic acid (GlcA) and Fucose (Fuc) residues with different molar ratios, while FS2 content only fucose residues. Sulfate contents of FCS1 and FS2 were 47.4% and 48.1%, respectively. FCS1 and FS2 fractions were different in the pattern of sulfation of  N-Acetyl-Galactosamine and fucose residues. IR and NMR spectra of two frations showed that sulfate groups were primarily occupied at C4 of pyranose residues in FS2 and C6, C2 and/or C3 of pyranose residues in FCS1 fraction. Our results contributed to knowledge on structural types of glycosaminoglycan from sea cucumbers in Vietnam. The establishment of structural features plays an important role in further studies of the structure-bioactivity relationship of sea cucumber glycosaminoglycan.


Glycosaminoglycans, fucosylated chondroitin sulfate, fucan sulfate, sea cucumber, Stichopus horrens

Full Text:



Chen SG, Xue CH, Yin LA, Tang QJ, Yu GL, Chai WG. Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers, Carbohydr Polym, 83, (2011), 688-896.

Dong X, Pan R, Deng X, Chen Y, Zhao G, Wang C. Separation purification, anticoagulant activity and preliminary structural characterization of two sufated polysaccharides from sea cucumber Acaudina molpadioidea and Holothuria nobilis, Process Biochemistry, 49, (2014), 1352-1361.

Wu. N. A, Chen. S. G, Ye. X. Q, Li. G. Y, Yin. L, & Xue. C. H. Identification of fucans from four species of sea cucumber by high temperature 1H-NMR, Journal of Ocean University of China, 13(5), (2014), 871-876.

Nadezhda E. Ustyuzhanina, Maria I. Bilan, Andrey S. Dmitrenok, Elizaveta Yu. Borodina, Nikolay E. Nifantiev, Anatolii I. Usov. A highly regular fucan sulfate from the sea cucumber Stichopus horrens, Carbohydrate Research, 456, (2018), 399-405.

Mingyi Wu, Li Xu , Longyan Zhao, Chuang Xiao, Na Gao, Lan Luo, Lian Yang , Zi Li , Lingyun Chen and Jinhua Zhao. Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber, Mar. Drugs, 13, (2015),2063-2084

Kariya, Y., Mulloy, B., Imai, K., Tominaga, A., Kaneko, T., Asari, A., et al. Isolation and partial characterization of fucan sulfateses from the body wall of sea cucumber Stichopus japonicus and their ability to inhibit osteoclastogenesis, Carbohydrate Research, 339 (7), (2004), 1339-1346.

Long Yu, Changhu Xue, Yaoguang Chang, Xiaoqi Xu, Lei Ge , Guanchen Liu, Yanchao Wang. Structure elucidation of fucoidan composed of a novel tetrafucose repeating unit from sea cucumber Thelenota ananas, Food Chemistry 146, (2014), 113–119

Đào Tấn Hổ (1991), Nguồn lợi Hải sâm (Holothuroidea) ở vùng biển phiá Nam Việt Nam. Tuyển tập báo cáo khoa học toàn quốc về biển lần thứ III.

Pham Duc Thinh, Bui Minh Ly, Roza V. Usoltseva, Natalia M.Shevchenko, Anton B. Rasin, Stanislav D. Anastyuk, Olesya S.Malyarenko, Tatiana N. Zvyagintseva, Pham Trung San, Svetlana, P. Ermakova. A novel sulfated fucan from Vietnamese sea cucumber Stichopus variegatus: Isolation, structure and anticancer activity in vitro. International Journal of Biological Macromolecules, 117, (2018), 1101–1109.

Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. Colorimetric method for determination of sugars and related substances, Analytical Chemistry, 28, (1956), 350-356.

Dodgson. K. S, Price. R. G. A. A note on the Determination of the Ester Sulphate Content of Sulphated Polysaccharides, Biochem. J, 84, (1962), 106-110.

O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem, 193, (1951), 265-275.

T. Bitter, H.M. Muir, A modified uronic acid carbazole reaction, Anal. Biochem. 4, (1962) 330-334.

Myron, P., Siddiquee, S., & Azad, S. A. (2017). Partial structural studies of fucosylated chondroitin sulfate (FuCS) using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and chemometrics. Vibrational Spectroscopy, 89, 26–36.

Nadezhda E. Ustyuzhanina, Maria I. Bilan, Andrey S. Dmitrenok, Eugenia A. Tsvetkova, Alexander S. Shashkov, Valentin A. Stonik, Nikolay E. Nifantiev, Anatolii I. Usov. Structural characterization of fucosylated chondroitin sulfates from sea cucumbers Apostichopus japonicus and Actinopyga mauritiana, Carbohydrate Polymers, 153, (2016), 399-405.

Lan Luo, Mingyi Wu, Li Xu, Wu Lian, Jingying Xiang, Feng Lu, Na Gao, Chuang Xiao, Shengmin Wang, and Jinhua Zhao. Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers, Mar. Drugs, 11, (2013), 399-417

Mohamed Ben Mansour, Rafik Balti, Véronique Ollivier, Hichem Ben Jannet, Frédéric Chaubet, Raoui Mounir Maaroufi. Characterization and anticoagulant activity of a fucosylated chondroitin sulfate with unusually procoagulant effect from sea cucumber, Carbohydrate Polymers, 174, (2017), 760–771.

Dongda Yang, Fudi Lin, Yayan Huang, Jing Ye, Meitian Xiao. Separation, purification, structural analysis and immune-enhancing activity of sulfated polysaccharide isolated from sea cucumber viscera, International Journal of Biological Macromolecules, 155, (2020) 1003–1018

Qiang Lia, Shuxin Jianga, Weiwei Shia, Xiaohui Qi, Weiguo Song, Jiaojiao Moua, Jie Yang. Structure characterization, antioxidant and immunoregulatory properties of a novel fucoidan from the sea cucumber Stichopus chloronotus, Carbohydrate Polymers, 231 (2020) 115767

Ustyuzhanina N.E., Bilan M.I., Dmitrenok A.S., Shashkov A.S., Nifantiev N.E., Usov A.I. Two structurally similar fucosylated chondroitin sulfates from the holothurian species Stichopus chloronotus and Stichopus horrens. Carbohydr. Polym, 189, (2018), 10-14.

Pereira, M.S., Oehninger,S., Barnett,T., Williams,R.L. and Clark, G. F. Structure and anticoagulant activity of sulfate fucans. Comparision between the regular, repetirive, and liner fucans from echinoderms with the more heterogeneous and branches polymers from brow algae. Journal of Biological Chemistry, 247 (12), (1993), 7656-7667.

DOI: Display counter: Abstract : 19 views. PDF : 8 views.


  • There are currently no refbacks.

Bioteknologi Agrikultur

Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology