Hung Huy Nguyen, Dai Ngoc Do, Prabodh Satyal, Chung Thanh Nguyen, Nguyen Van Bui, William N Setzer


Abstract – HCTN7. The leaves of Vitex negundo L. (Verbenaceae) were collected from Dan Nang City and the Pu Hoat Nature Reserve, Nghe An province, and were hydrodistilled to give the essential oils.  The leaf oils (six separate samples) were analyzed by gas chromatographic – mass spectrometric methods, and showed very different chemical compositions. The major components in the four essential oils from Da Nang were sabinene (6.0-19.9%), 1,8-cineole (1.6-13.7%), α-terpinyl acetate (1.9-7.8%), (E)-caryophyllene (5.7-18.3%), eremophilene (13.1-33.6%), caryophyllene oxide (4.9-18.1%), and an unidentified diterpenoid (5.2-8.3%); while the major components in the two Pu Hoat samples were sabinene (14.7% and trace), trans-β-elemene (0.2% and 11.1%), (E)-caryophyllene (57.0% and 48.2%), and caryophyllene oxide (5.4% and 3.2%).  One sample of V. negundo leaf essential oil from Da Nang and one from Pu Hoat were screened for larvicidal activity against Aedes aegypti, a vector of dengue fever, chikungunya, Zika fever, Mayaro and yellow fever viruses.  The Da Nang essential oil showed only marginal larvicidal activity (24-h LC50 = 82.9 μg/mL; 48-h LC50 = 72.2 μg/mL), but the Pu Hoat sample was significantly more active (24-h LC50 = 16.8 μg/mL; 48-h LC50 = 14.2 μg/mL).  As a test for pesticidal selectivity, V. negundo leaf oil from Pu Hoat was also screened against the water bug, Diplonychus rusticus, an insect predator of mosquito larvae.  The essential oil was significantly less toxic to D. rusticus (24-h LC50 = 136 μg/mL; 48-h LC50 = 134 μg/mL).


Verbenaceae, Vitex negundo, Aedes aegypti, arbovirus, dengue fever

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