Chinh Thuy Nguyen, Hung Thi Pham, Hoang Thai


This work is focused on preparation and characterization of chitosan/alginate/ginsenoside Rb1 nanoparticles prepared by ionic gelation method with ginsenoside Rb1 content of 3, 5 and 7 wt.% in comparison with total weight of chitosan and alginate. Some methods including to Infrared Spectroscopy (IR), Dynamic Light Scattering (DLS), and Field Emission Scanning Electron Microscopy (FESEM) and Ultraviolet-Visible spectroscopy (UV-Vis) methods were used to determine the functional groups, size distribution, morphology and ginsenoside Rb1 release content from the chitosan/alginate/ginsenoside Rb1 nanoparticles. Ginsenoside Rb1 was dispersed regularly in chitosan/alginate matrix. The particle size of chitosan/alginate/ginsenoside Rb1 nanoparticles was reduced as increasing the ginsenoside Rb1 content. The ginsenoside Rb1 release content from the chitosan/alginate/ginsenoside Rb1 nanoparticles in simulated body fluids was also evaluated and discussed.


ginsenoside Rb1; functional groups; size distribution; drug release

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Kim H. K. - Pharmacokinetics of ginsenoside Rb1 and its metabolite compound K after oral administration of Korean Red Ginseng extract, J. Ginseng Res., 37 (4) (2013) 451-456.

Hwang J. Y., Shim J. S., Song M. Y., Yim S. V., Lee S. E., Park K. S. - Proteomic analysis reveals that the protective effects of ginsenoside Rb1 are associated with the actin cytoskeleton in β-amyloid-treated neuronal cells, J. Ginseng Res. 40 (2016) 278–284.

Christensen L. P. - Ginsenosides: chemistry, biosynthesis, analysis, and potential health effect, Adv. Food Nutr. Res. 55 (2009) 1–99.

Liang W., Ge S., Yang L., Yang M., Ye Z., Yan M., Du J., Luo Z. - Ginsenosides Rb1 and Rg1 promote proliferation and expression of neurotrophic factors in primary Schwann cell cultures, Brain Res. 1357 (2010) 19–25.

Yu-Hai G., Shuai Z., Yan-Xin D., Qing-Jia X., Bo-Lai C., Chu-Qin Y. - Effects of ginsenoside Rg1-loaded alginate-chitosan microspheres on human bone marrow stromal cells, Biosci. Rep. 37 (3) (2017) BSR20160566.

Zhang J., Wang Y., Jiang Y., Liu T., Luo Y., Diao E., Cao Y., Chen L., Zhang L., Gu Q., Zhou J., Sun F., Zheng W., Liu J., Li X., Hu W. - Enhanced cytotoxic and apoptotic potential in hepatic carcinoma cells of chitosan nanoparticles loaded with ginsenoside compound K, Carbohydr. Polym. 198 (2018) 537-545

Mathiyalagan R., Subramaniyam S., Kim Y. J., Kim Y. C., Yang D. C. - Ginsenoside compound K-bearing glycol chitosan conjugates: synthesis, physicochemical characterization, and in vitro biological studies, Carbohydr. Polym. 112 (2014) 359-366.

Yang L., Zhang Z., Hou J., Jin X., Ke Z., Liu D., Du M., Jia X., Lv H. - Targeted delivery of ginsenoside compound K using TPGS/PEG-PCL mixed micelles for effective treatment of lung cancer, Int. J. Nanomedicine 12 (2017) 7653-7667.

Dong Y., Fu R., Yang J., Ma P., Liang L., Mi Y., Fan D. - Folic acid-modified ginsenoside Rg5-loaded bovine serum albumin nanoparticles for targeted cancer therapy in vitro and in vivo, Int. J. Nanomedicine 14 (2019) 6971-6988.

Marguerite R. - Chitin and chitosan: Properties and applications, Progress in Polymer Science 31 (7) (2006) 603-632.

Draget K. I. and Taylor C. - Chemical, physical and biological properties of alginates and their biomedical implications, Food Hydrocoll. 25 (2011) 251–256.

Kuen Y. L., David J. M. - Alginate: Properties and biomedical applications, Progress in Polymer Science 37 (1) (2012) 106-126.

Sun X., Shi J., Xu X., Cao S. - Chitosan coated alginate/poly(N-isopropylacrylamide) beads for dual responsive drug delivery, Int. J. Biol. Macromol. 59 (2013) 273–281.

Zhang Y., Wei W., Lv P., Wang L., Ma G. - Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin, Eur. J. Pharm. Biopharm. 77 (1) (2011) 11-19.

Masalova O., Kulikouskaya V., Shutava T., Agabekov V. - Alginate and chitosan gel nanoparticles for efficient protein entrapment, Physics Procedia 40 (2013) 69 – 75.

Hye-Lee K., Gil-Yong J., Jun-Ho Y., Jung-Suk H., Yoon-Jeong P., Do-Gyoon K., Miqin Z., Dae-Joon K. - Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering, Mater. Sci. Eng. C 54 (2015) 20-25.

Hoang T., Kavitha R., Loc T. T., Mai T. T., Giang L. D., Thang V. V., Tuan T. M., Chinh N. T. - Novel drug delivery system based on ginsenoside Rb1 loaded to chitosan/alginate nanocomposite films, J. Nanosci. Nanotechnol. 19 (6) (2019) 3293-3300.

Hoang T., Lam T. D., Loc T. T., Giang L. D., Trang T. D. M., Trung V. Q., Anh N. T., Trinh N. D., Chinh N. T. - Effect of both lovastatin and ginsenoside Rb1 on some properties and in-vitro drug release of alginate/chitosan/lovastatin/ginsenoside Rb1 composite films, J. Polym. Environ. 27 (12) (2019) 2728-2738.

Loc T. T., Chinh N. T., Quan V. A., Thanh D. M., Tung N. Q., Anh N. T., Giang . L., Hoang T. - Assessment of the role of ginsenoside Rb1 active substance in alginate/chitosan/lovastatin composite films, Int. J. Polym. Sci. 2020 (2020) 11 pages.

Nafisa G., Shahzad M. K., Muhammad T. Z. B., Syed K., Muhammad S., Atif I., Sumreen A., Sadaf H., RafiUllah K. - In vitro study of chitosan-based multi-responsive hydrogels as drug release vehicles: a preclinical study, RSC Adv. 9 (2019) 31078.

Loreana L., Alexandre L. P., Valfredo F., Mauro C. M. L., Hellen K. S. - Development and evaluation of pH-sensitive sodium alginate/chitosan microparticles containing the antituberculosis drug rifampicin, Mater. Sci. Eng. C 39 (2014) 161–167.

Cui-Yun Y., Xi-Chen Z., Fang-Zhou Z., Xian-Zheng Z. - Sustained release of antineoplastic drugs from chitosan-reinforced alginate microparticle drug delivery systems, Inter. J. Pharmaceut. 357 (2008) 15 – 21.

Ya-Ni D., P. L., Jun-Ping Z., Wang A., Wei Q. - Swelling characteristics and drug delivery properties of nifedipine-loaded pH sensitive alginate-chitosan hydrogel beads, J. Biomed. Mater. Res. B, Appl. Biomater. 86 (2) (2008) 493-500.

Wang F. Q., Li P., Zhang J. P., Wang A. Q., Wei Q. - pH-sensitive magnetic alginate-chitosan beads for albendazole delivery, Pharm. Dev. Technol.16 (3) (2011) 228-236.

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