ISOLATION OF MARINE BACTERIA FROM SPONGES IN THE SOUTH-CENTRAL COASTAL REGION OF VIETNAM WITH BROWN SEAWEED POLYSACCHARIDE-DEGRADING ACTIVITIES

TRANG VO THI DIEU, TRANG VO THI DIEU, HANG CAO THI THUY HANG, TRINH PHAN THI HOAI, NGOC NGO THI DUY, KHANH HUYNH HOANG NHU, VAN TRAN THI THANH

Abstract


Oligosaccharides, transformed products of seaweed polysaccharides, have been reported to have valuable biological activities, in which they act as so they are potential compounds for biomaterials, biofuels, and nutrients, cosmetic and medical fields. Polysaccharide-degrading enzymes are the best tools for the preparation of these bioactive oligosaccharides. The potential of producing brown seaweed polysaccharide-degrading enzymes of marine bacteria associated with sponges from Phu Quy Island and Van Phong Bay, Vietnam was conducted on alginate and fucoidan extracted from brown seaweeds. In that, screening for producing alginate lyase by using alginate prepared from Sargassum mcclurei (S. mcc A), and producing fucoidanase by using fucoidan prepared from S. mcclurei (S. mcc F), S. polycystum (S. poly F) and S. oligocystum (S. oli F) as reference substrates. The results in this present study showed that thirty-five bacterial strains were isolated from twenty sponge samples in both positions. And the proportion of positive isolated bacterial strains on S. mcc A, S. mcc F, S. poly F, and S. oli F substrates were 68.6%, 34.3%, 48.6%, and 20%, respectively. Analyzing 16S rRNA sequences of four selected bacterial strains, which exhibited a wide spectrum of enzymatic activities, 1911PQ-1.37.6, 1911PQ-1.37.2, 1911VP-3.52.6, and 1911VP-3.28.2 showed substantial similarity (higher 99.5% of percent identity and 100% query cover) with Staphylococcus arlettae, S. pasteuri, Bacillus megaterium and, Alteromonas macleodii, respectively. These investigations demonstrated the potential of bacterial associated with sponges in the south-central coastal region of Vietnam Sea for the explorations of novel seaweed polysaccharide-degrading enzymes.


Keywords


Alginate lyase; bacteria; fucoidanase; seaweed polysaccharides; sponges;

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DOI: https://doi.org/10.15625/2525-2518/58/6A/15445 Display counter: Abstract : 19 views. PDF : 6 views.

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