Thu Thuy Thi Tran, Ha Thi Dinh, Baskar Salvaraja, Long Quoc Pham, Quang Dai Ngo, Nghi Huu Do, Jae Wook Lee


A simple and efficient synthesis of two new hydroximinosteroidal derivatives (11, 13) from cholesterol was described. The cytotoxicity of these compounds, their intermediates were evaluated on human cancer cell lines including Hep-G2, HeLa and T98G. Compound 13 presented a potent antiproliferative effect to T98G cancer cell at 2.9 mM of IC50. In addition, the antimicrobial assay showed that two new oximes 11 and 13 were active against E. coli and S. aureus strain (MIC :50 mg/mL).


cholesterol, hydroximinosteroid, HeLa, HepG2, T98G

Full Text:



Sarma N. S., Krishna M. S. R., Rao S. R. - Sterol ring system oxidation pattern in marine sponges, Marine Drugs, 3 (2005), 84-111.

Ivanchina N. V., Kicha A. A., Stonik V. A. – Steroid glycosides from marine organisms, Steroids, 76 (2011), 425-454.

Rodriguez J., Nunez L., Pexinho S., Jimenez C. – Isolation and synthesis of the first natural 6-hydroximino-4-en-3-one steroids from the sponges Cinachyrella spp., Tet. Lett. 38 (1997), 1833-1836.

Xiao D. J., Peng X. D., Deng S. Z., Ma W. Z., Wu H. M. – Structure elucidation of (3E)-cholest-4-en-3,6-dione-3-oxime in marine sponge Cinachyrella australiensis from the south china sea, Chin. J. Org. Chem. 25 (2005), 1606-1609.

Cui J. G., Huang L., Fan L., Zhou M. A. - A facile and efficient synthesis of some (6E)-hydroximino-4-en-3-one steroids, steroidal oximes from Cinachyrella sp. Sponges, Steroids, 73 (2008), 252-256.

Noé Deive, Jaime Rodríguez, and Carlos Jiménez – Synthesis of cytotoxic 6E-Hydroximino-4-ene steroids: Structure/Activity studies, J. Med. Chem. 44 (2001), 2612-2618.

Cui J. G., Fan L., Huang L., Liu L., Zhou M. A. – Synthesis and evaluation of some steroidal oximes as cytotoxic agents: Structure/activity studies (I), Steroids 74 (2009), 62-72.

Cui J. G., Fan L., Huang M. Y., Xin Y., Zhou M. A. - Synthesis and evaluation of some steroidal oximes as cytotoxic agents: Structure/activity studies (II), Steroids 74 (2009), 989-995.

Javier P., Miriam R., Vanessa P., Beatriz A., Jaime R., Nelida S., Antonio F., Carlos J. – Synthesis and evaluation of new 6-hydroximinosteroids analogs as cytotoxic agents, Bioorg. Med. Chem. 15 (2007), 4722-4740.

D.A. Vanden Bergher, A. J. Vlietinck. Screening Methods of Antibacterial and Antiviral Agents from Higher Plants. Methods in Plant biochemistry. Academic Press, USA (1991), V. 6.

Do Huu Nghi, Tran Thi Nhu Hang, Vu Dinh Giap, Do Thi Nhuan - Screening for antiproliferative and antimicrobial activity of total lipids of some marine invertebrates collected from Vietnam’s North Central Coast. Vietnam J. Chem. 55 (2017), 124-130.

Mosman T., - Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J. Immunol. Methods, 65 (1983), 55-63.

Cui J. G., Cui W. L., Long M. Z., Jing Y. S. - Synthesis of polyhydroxysterols (III): synthesis and structural elucidation of 24-methylenecholest-4-en-3β,6α-diol, Steroids 67 (2002), 1015-1019.

DOI: Display counter: Abstract : 420 views. PDF : 175 views.


  • There are currently no refbacks.

budidaya tani

Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology