Identification and characterization of a purple nonsulfur bacterium isolated from coastal area of Hai Phong for using in production of unsaturated fatty acid (omega 6, 7, 9)

Hoang Thi Yen, Tran Thi Thu Quynh, Chu Hoang Ha, Do Thi Tuyen, Dang Tat Thanh, Dinh Thi Thu Hang
Author affiliations

Authors

  • Hoang Thi Yen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam
  • Tran Thi Thu Quynh Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam
  • Chu Hoang Ha Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam
  • Do Thi Tuyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam
  • Dang Tat Thanh Department of Science and Technology, Ministry of Industry and Trade, 54 Hai Ba Trung Road, Hoan Kiem District, Ha Noi, Viet Nam
  • Dinh Thi Thu Hang Graduate University of Sciences and Technology, VAST, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/57/6/13711

Abstract

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.

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Published

20-11-2019

How to Cite

[1]
H. T. Yen, T. T. T. Quynh, C. H. Ha, D. T. Tuyen, D. T. Thanh, and D. T. T. Hang, “Identification and characterization of a purple nonsulfur bacterium isolated from coastal area of Hai Phong for using in production of unsaturated fatty acid (omega 6, 7, 9)”, Vietnam J. Sci. Technol., vol. 57, no. 6, pp. 665–676, Nov. 2019.

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