High performance liquid chromatography assay for determination of moxifloxacin in human plasma

Luong Thi My Hanh, Nguyen Thi Minh Diep, Pham Thi Ngoc Mai, Nguyen Xuan Truong
Author affiliations

Authors

  • Luong Thi My Hanh Faculty of Chemistry, Hanoi University of Science, VNU Ha Noi, 19 Le Thanh Tong street, Hoan Kiem, Ha Noi, Viet Nam
  • Nguyen Thi Minh Diep Department of Drug Quality Control, Phu Tho Medical and Pharmacy College, No. 2201, Hung Vuong street, Viet Tri, Phu Tho, Viet Nam
  • Pham Thi Ngoc Mai Faculty of Chemistry, Hanoi University of Science, VNU Ha Noi, 19 Le Thanh Tong street, Hoan Kiem, Ha Noi, Viet Nam
  • Nguyen Xuan Truong School of Chemical Engineering, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/57/3/13362

Keywords:

Moxifloxacin, protein precipitation, tricloroacetic acid, HPLC method, human plasma

Abstract

A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.

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Published

04-06-2019

How to Cite

[1]
L. T. M. Hanh, N. T. M. Diep, P. T. Ngoc Mai, and N. X. Truong, “High performance liquid chromatography assay for determination of moxifloxacin in human plasma”, Vietnam J. Sci. Technol., vol. 57, no. 3, pp. 336–343, Jun. 2019.

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Section

Materials