Luong Thi My Hanh, Nguyen Thi Minh Diep, Pham Thi Ngoc Mai, Nguyen Xuan Truong


A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.


Moxifloxacin, protein precipitation, tricloroacetic acid, HPLC method, human plasma

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(1) R. Ronald, A.; Low, D. Fluoroquinolone Antibiotics.; Birkhauser Boston Springer: Cambridge; Secaucus, 2012.

(2) The Quinolones, 3rd ed.; Andriole, V. T., Ed.; Academic Press: San Diego, 2000.

(3) Appelbaum, P. C.; Hunter, P. A. The Fluoroquinolone Antibacterials: Past, Present and Future Perspectives. Int. J. Antimicrob. Agents 2000, 16 (1), 5–15.

(4) Labandjurdjevic, A.; Jelikicstankov, M.; Djurdjevic, P. Optimization and Validation of the Direct HPLC Method for the Determination of Moxifloxacin in Plasma. J. Chromatogr. B 2006, 844 (1), 104–111. https://doi.org/10.1016/j.jchromb.2006.07.001.

(5) Tatar Ulu, S. High-Performance Liquid Chromatography Assay for Moxifloxacin: Pharmacokinetics in Human Plasma. J. Pharm. Biomed. Anal. 2007, 43 (1), 320–324. https://doi.org/10.1016/j.jpba.2006.06.026.

(6) Hemanth Kumar, A. K.; Ramachandran, G. Simple and Rapid Liquid Chromatography Method for Determination of Moxifloxacin in Plasma. J. Chromatogr. B 2009, 877 (11–12), 1205–1208. https://doi.org/10.1016/j.jchromb.2009.02.042.

(7) Hurtado, F. K.; Kaiser, M.; Tasso, L.; Dalla Costa, T. Fast and Sensitive RP-HPLC—Fluorescence Method for the Quantitative Analysis of Moxifloxacin in Rat Plasma and Its Application to a Preclinical Pharmacokinetic Study. Acta Chromatogr. 2016, 28 (2), 175–191. https://doi.org/10.1556/1326.2016.28.2.3.

(8) Khan, F. U.; Nasir, F.; Iqbal, Z.; Khan, I.; Shahbaz, N.; Hassan, M.; Ullah, F. Simultaneous Determination of Moxifloxacin and Ofloxacin in Physiological Fluids Using High Performance Liquid Chromatography with Ultraviolet Detection. J. Chromatogr. B 2016, 1017–1018, 120–128. https://doi.org/10.1016/j.jchromb.2016.03.002.

(9) Czyrski, A.; Sokół, A.; Szałek, E. HPLC Method for Determination of Moxifloxacin in Plasma and Its Application in Pharmacokinetic Analysis. J. Liq. Chromatogr. Relat. Technol. 2017, 40 (1), 8–12. https://doi.org/10.1080/10826076.2017.1280680.

(10) Wang, N.; Zhu, L.; Zhao, X.; Yang, W.; Sun, H. Improved HPLC Method for the Determination of Moxifloxacin in Application to a Pharmacokinetics Study in Patients with Infectious Diseases. ISRN Pharmacol. 2013, 2013, 1–7. https://doi.org/10.1155/2013/462918.

(11) Xu, Y. H.; Li, D.; Liu, X. Y.; Li, Y. Z.; Lu, J. High Performance Liquid Chromatography Assay with Ultraviolet Detection for Moxifloxacin: Validation and Application to a Pharmacokinetic Study in Chinese Volunteers. J. Chromatogr. B 2010, 878 (32), 3437–3441. https://doi.org/10.1016/j.jchromb.2010.10.024.

(12) Trần Cao Sơn. Thẩm Định Phương Pháp Trong Phân Tích Hóa Học & vi Sinh Vật; Nhà xuất bản Khoa học và Kỹ thuật, 2010.

DOI: https://doi.org/10.15625/2525-2518/57/3/13362 Display counter: Abstract : 373 views. PDF : 119 views.


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