SEPARATION AND HPLC QUANTITATIVE ANALYSIS OF MURRAYAFOLINE A FROM THE ROOTS OF GLYCOSMIS STENOCARPA

Tran Quoc Toan

Abstract


In this study, we investigated two methods for the separation and purification of alkaloid
Murrayafoline A from the roots of the Vietnamese plant Glycosmis stenocarpa and developed an
HPLC method for the determination of its contents in plant materials and the methanol extract.
The yield of Murrayafoline A using steam distillation method was 1.5 times higher than the
separation method using column chromatography. By HPLC method, the content of
Murrayafoline A was determined to be 0.38 % (w/w) in the roots and 16.69 % (w/w) in the
methanol extract of G. stenocarpa.


Keywords


Glycosmis stenocarpa, Rutaceae, carbazole alkaloid, Murrayafoline A.

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DOI: https://doi.org/10.15625/2525-2518/54/2C/11884 Display counter: Abstract : 40 views. PDF : 76 views.

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Published by Vietnam Academy of Science and Technology