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EFFECT OF DIETARY ASTAXANTHIN ON PIGMENT ACCUMULATION IN THE MUSCLE-SKIN, RESISTANCE TO SALINITY STRESS AND COPPER TOXICITY OF COMMERCIAL CLOWNFISH, Amphiprion ocellaris

Ho Son Lam, Phan Thi Ngoc

Abstract


This study examined the influence of Astaxanthin added in feed on pigment accumulation in the muscle-skin, as well as resistance to salinity stress and copper toxicity of clownfish (Amphiprion ocellaris). Mean length of fish was 33.05 ± 3.29 mm. Five experiments were performed with synthetic Astaxanthin contents: 0, 50, 100, 150 and 200 mg/kg diets. After 8 weeks of the experiment, the pigment accumulation of Astaxanthin in the muscle-skin of clownfish was the lowest in the control treatment of 2.11 ± 0.13 µg/g, while these treatments of 150 and 200 mg/kg diets were over 10 µg/g. The treatments of 150 and 200 mg Astaxanthin/kg increased the resistance to copper toxicity and salinity stress test of the fish. The resistance of the fish was longer (>50 minutes) compared to the control (42.4 minutes) in an environment with salinity of 0‰ (p<0.05); The mortality was below 68% instead of 100% in the control after 5 and 7 days of culture in the environment 5‰ and 10‰, respectively (p<0.05). The survival rate of fish was over 75%, respectively, significantly higher than the control (54%) when being cultured in the environment of Cu2+ - 96 h (p<0.05). However, there were no significant differences in accumulation of Astaxanthin and resistance to the stress test in two treatments of 150, 200 mg/kg diets (p>0.05). Thus, the optimal content of astaxanthin supplement in fish food was 150 mg/kg diet, which created high pigment accumulation in the muscle-skin; increased resistance to salinity stress and copper toxicity.

Keywords


Astaxanthin, clownfish, pigment, salinity stress, copper stress.

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References


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DOI: https://doi.org/10.15625/1859-3097/18/1/8940

Journal of Marine Science and Technology ISSN: 1859 3097

Published by Vietnam Academy of Science and Technology