Bacillus sp. VK2 ISOLATED FROM Acropora hyacinthus FROM NINH THUAN AND ITS ANTIMICROBIAL ACTIVITIES AGAINST CAUSE OF WHITE POX DISEASE IN Acropora palmate

Pham Thi Mien, Nguyen Ngoc Thang, Nguyen Kim Hanh
Author affiliations

Authors

  • Pham Thi Mien Institute of Oceanography-VAST
  • Nguyen Ngoc Thang Institute of Oceanography, VAST
  • Nguyen Kim Hanh Institute of Oceanography, VAST

DOI:

https://doi.org/10.15625/1859-3097/18/2/8766

Keywords:

Antimicrobial activity, Bacillus sp. VK2, Acropora hyacinthus, Ninh Thuan.

Abstract

Coral associated bacteria and their role for the host are currently one of the interested issues for research and scientists worldwide. Search of associated bacteria with some species of hard coral in Hang Rai, Ninh Thuan found that the strain VK2 was the most abundant culture from Acropora hyacinthus. This bacterium was implicated to further study for antimicrobial tests with Bacillus subtilis ATCC6633, Salmonella typhimurium ATCC6994, Escherichia coli O157, and Serratia marcescens PDL100 by agar diffusion method on MHA. The bacterium was identified as Bacillus sp. VK2. This strain showed inhibition of Serratia marcescens strain PDL100-as pandemic white pox disease for elkhorn coral Acropora palmata in Florida Keys, USA. However Bacillus sp. strain VK2 showed no inhibition against other indicators.

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References

Kushmaro, A., Rosenberg, E., Fine, M., and Loya, Y., 1997. Bleaching of the coral Oculina patagonica by Vibrio AK-1. Marine Ecology Progress Series, 147, 159-165.

Ben-Haim, Y., and Rosenberg, E., 2002. A novel Vibrio sp. pathogen of the coral Pocillopora damicornis. Marine Biology, 141(1), 47-55.

Ben-Haim, Y., Zicherman-Keren, M., and Rosenberg, E., 2003. Temperature-regulated bleaching and lysis of the coral Pocillopora damicornis by the novel pathogen Vibrio coralliilyticus. Applied and Environmental Microbiology, 69(7), 4236-4242.

Frias-Lopez, J., Zerkle, A. L., Bonheyo, G. T., and Fouke, B. W., 2002. Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. Applied and environmental microbiology, 68(5), 2214-2228.

Denner, E. B., Smith, G. W., Busse, H. J., Schumann, P., Narzt, T., Polson, S. W., ... and Richardson, L. L., 2003. Aurantimonas coralicida gen. nov., sp. nov., the causative agent of white plague type II on Caribbean scleractinian corals. International Journal of Systematic and Evolutionary Microbiology, 53(4), 1115-1122.

Holden, C., 1996. Coral disease hot spot in Florida Keys.

Science, 274(5295), 2017.

Miller, S., Chiappone, M., Rutten, L. M., and Swanson, D. W., 2008. Population status of Acropora corals in the Florida Keys.

Patterson, K. L., Porter, J. W., Ritchie, K. B., Polson, S. W., Mueller, E., Peters, E. C., Santavy, D. L., and Smith, G. W., 2002. The etiology of white pox, a lethal disease of the Caribbean elkhorn coral, Acropora palmata. Proceedings of the National Academy of Sciences, 99(13), 8725-8730.

Sutherland, K. P., Porter, J. W., Turner, J. W., Thomas, B. J., Looney, E. E., Luna, T. P., Meyers, M. K., Futch, J. C., and Lipp, E. K., 2010. Human sewage identified as likely source of white pox disease of the threatened Caribbean elkhorn coral, Acropora palmata. Environmental

Microbiology, 12(5), 1122-1131.

Sutherland, K. P., Shaban, S., Joyner, J. L., Porter, J. W., and Lipp, E. K., 2011. Human pathogen shown to cause disease in the threatened eklhorn coral Acropora palmata. PloS One, 6(8), e23468.

Polson, S. W., Higgins, J. L., and Woodley, C. M., 2008. PCR-based assay for detection of four coral pathogens. Proc. 11th Int. Coral Reef Symp., Ft Lauderdale, FL, 251-255.

Lesser, M. P., and Jarett, J. K., 2014. Culture-dependent and culture-independent analyses reveal no prokaryotic community shifts or recovery of Serratia marcescens in Acropora palmata with white pox disease. FEMS Microbiology Ecology, 88(3), 457-467.

Joyner, J. L., Sutherland, K. P., Kemp, D., Berry, B., Griffin, A., Porter, J., Amador, M. H. B., Noren, H. K. G., and Lipp, E. K., 2015. Systematic analysis of white pox disease in Acropora palmata of the Florida Keys and the role of Serratia marcescens. Applied and Environmental Microbiology, AEM-00116.

15. Koh, E. G., 1997. Do scleractinian corals engage in chemical warfare against microbes?. Journal of Chemical Ecology, 23(2), 379-398.

Geffen, Y., and Rosenberg, E., 2005. Stress-induced rapid release of antibacterials by scleractinian corals. Marine Biology, 146(5), 931-935.

Leruste, A., Bouvier, T., and Bettarel, Y., 2012. Enumerating Viruses in Coral Mucus. Applied and Environmental Microbiology, 78(17), 6377-6379.

Halebian, S., Harris, B., Finegold, S. M., and Rolfe, R. D., 1981. Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. Journal of Clinical Microbiology, 13(3), 444-448.

Holt, J. G., and Sneath, P. H., 1984. Bergey’s Manual of Systematic Bacteriology. Baltimore, London. Vol 2.

Bauer, A. W., Kirby, W. M. M., Sherris, J. C., and Turck, M., 1966. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4_ts), 493-496.

Team, R. C., 2012. R: A language and environment for statistical computing. http://www. R-project. org.

Phạm Thị Miền, Võ Hải Thi, Lê Hoài Hương và Hoàng Xuân Bền, 2010. Phân lập vi khuẩn từ san hô mềm Sinularia spp. và thử nghiệm hoạt tính kháng Tetracycline, Gentamicin, Cefazolin của chúng. Tuyển Tập nghiên cứu biển, Tập XVII, 183-195.

Hanh, N. K., Bettarel, Y., Bouvier, T., Bouvier, C., Hai, D. N., Lam, N. N., Thuy N. T., Huy T. Q., and Brune, J., 2015. Coral mucus is a hot spot for viral infections. Applied and Environmental

Microbiology, 81(17), 5773-5783.

Nguyen‐Kim, H., Bouvier, T., Bouvier, C., Doan‐Nhu, H., Nguyen‐Ngoc, L., Rochelle‐Newall, E., Baudoux, C. A., Desnues, C., Reynaud, S., Ferrier‐Pages, S., and Bettarel, Y., 2014. High occurrence of viruses in the mucus layer of scleractinian corals. Environmental Microbiology

Reports, 6(6), 675-682.

Nithyanand, P., and Pandian, S. K., 2009. Phylogenetic characterization of culturable bacterial diversity associated with the mucus and tissue of the coral Acropora digitifera from the Gulf of Mannar. FEMS Microbiology Ecology, 69(3), 384-394.

De Castro, A. P., Araújo, S. D., Reis, A. M., Moura, R. L., Francini-Filho, R. B., Pappas, G., Rodrigues, T. G., Thompson, F. L., and Krüger, R.

H., 2010. Bacterial community associated with healthy and diseased reef coral Mussismilia hispida from eastern Brazil. Microbial

Ecology, 59(4), 658-667.

Kvennefors, E. C. E., Sampayo, E., Kerr, C., Vieira, G., Roff, G., and Barnes, A. C., 2012. Regulation of bacterial communities through antimicrobial activity by the coral holobiont. Microbial

Ecology, 63(3), 605-618.

Simon, C., and Daniel, R., 2011. Metagenomic analyses: past and future trends. Applied and Environmental Microbiology, 77(4), 1153-1161.

Zhang, X., Sun, Y., Bao, J., He, F., Xu, X., and Qi, S., 2012. Phylogenetic survey and antimicrobial activity of culturable microorganisms associated with the South China Sea black coral Antipathes dichotoma. FEMS Microbiology Letters, 336(2), 122-130.

Peng, J., Zhang, X., Xu, X., He, F., and Qi, S., 2013. Diversity and chemical defense role of culturable non-actinobacterial bacteria isolated from the South China Sea (Bien Dong Sea) gorgonians. J. Microbiol Biotechnol, 23(4), 437-43.

Lampert, Y., Kelman, D., Dubinsky, Z., Nitzan, Y., and Hill, R. T., 2006. Diversity of culturable bacteria in the mucus of the Red Sea coral Fungia scutaria. FEMS microbiology ecology, 58(1), 99-108.

Ritchie, K. B., 2006. Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Marine Ecology Progress Series, 322, 1-14.

Shnit-Orland, M., and Kushmaro, A., 2009. Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiology

Ecology, 67(3), 371-380.

Shnit-Orland, M., Sivan, A., and Kushmaro, A., 2012. Antibacterial activity of Pseudoalteromonas in the coral holobiont. Microbial

Ecology, 64(4), 851-859.

Reshef, L., Koren, O., Loya, Y., Zilber‐Rosenberg, I., and Rosenberg, E., 2006. The coral probiotic hypothesis. Environmental

Microbiology, 8(12), 2068-2073.

Pham, T. M., Wiese, J., Wenzel-Storjohann, A., and Imhoff, J. F., 2016. Diversity and antimicrobial potential of bacterial isolates associated with the soft coral Alcyonium digitatum from the Baltic Sea. Antonie Van Leeuwenhoek, 109(1), 105-119.

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Published

30-06-2018

How to Cite

Mien, P. T., Thang, N. N., & Hanh, N. K. (2018). Bacillus sp. VK2 ISOLATED FROM Acropora hyacinthus FROM NINH THUAN AND ITS ANTIMICROBIAL ACTIVITIES AGAINST CAUSE OF WHITE POX DISEASE IN Acropora palmate. Vietnam Journal of Marine Science and Technology, 18(2), 197–204. https://doi.org/10.15625/1859-3097/18/2/8766

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