Density and nitrifying potential of indigenous bacterial community in mangrove and seagrass in the north of Vietnam

Le Thanh Huyen, Dao Thi Anh Tuyet, Le Minh Hiep, Nguyen Tien Dat, Ha Thi Binh, Do Trung Sy, Do Manh Hao

Abstract


Density and nitrification potential of indigenous microorganism in mangroves (Tien Yen - Quang Ninh and Bang La - Hai Phong) and seagrass (Ha Coi, Dam Ha - Quang Ninh and Tam Giang - Thua Thien Hue) in the north of Vietnam were evaluated through 4 sampling times in the dry and rainy seasons in the years of 2017-2019. The analytical results showed that the average density of nitrifying bacteria was 4.6 ± 1.8 × 102 MPN/ml, in which the density in mangroves tended to be higher than that in the seagrass beds (P < 0.05) in both the rainy and dry seasons. The average nitrifying rate was 2.7 ± 0.6 µgN/g wet soil/hour, in which the rate in mangroves tended to be higher than that in seagrass beds in the rainy season (P < 0.05). Nitrifying density and rate are not only correlated with substrate concentration but also with other environmental factors such as P-PO4, BOD5, total phosphate in sediment and total bacterial density.


Keywords


Nitrifying bacteria, density, speed, mangrove forest, seagrass beds, north of Vietnam.

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References


[1] de Jonge, V. N., Elliott, M., and Orive, E., 2002. Causes, historical development, effects and future challenges of a common environmental problem: eutrophication. In Nutrients and eutrophication in estuaries and coastal waters (pp. 1–19). Springer, Dordrecht. https://doi.org/10.1007/978-94 -017-2464-7_1.

[2] Magalhães, C. M., Joye, S. B., Moreira, R. M., Wiebe, W. J., and Bordalo, A. A., 2005. Effect of salinity and inorganic nitrogen concentrations on nitrification and denitrification rates in intertidal sediments and rocky biofilms of the Douro river estuary, Portugal. Water Research, 39(9), 1783–1794. https://doi.org/10.1016/j.watres.2005.03.008.

[3] Gross, A., Abutbul, S., and Zilberg, D., 2004. Acute and Chronic Effects of Nitrite on white shrimp, Litopenaeus vannamei, cultured in low‐salinity brackish water. Journal of the World Aquaculture Society, 35(3), 315–321. https://doi.org/10.1111/j.1749-7345.2004.tb00095.x.

[4] Schuler, D. J., 2008. Acute toxicity of ammonia and nitrite to white shrimp (L. vannamei) at low salinities. Master thesis, Virginia Polytechnic Institute and State University. 53 p.

[5] Kim, D. H., Matsuda, O., and Yamamoto, T., 1997. Nitrification, denitrification and nitrate reduction rates in the sediment of Hiroshima Bay, Japan. Journal of Oceanography, 53, 317–324.

[6] Doan Bo, 2001. Marine chemistry: Methods of analyzing sea water chemistry. Hanoi National University Publisher, 131 p.

[7] TCVN 6001-2:2008. Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 2: Method for undiluted samples.

[8] TCVN 6491-1999. Water quality - Determination of chemical oxygen demand.

[9] Nguyen Lan Dung, Doan Xuan Loan, Nguyen Phung Tien, Dang Duc Trach and Pham Van Ty, 1972. Some research methods of microbiology - Volume 2. Science and Technics Publishing House, Hanoi. 430 p.

[10] TCVN 6643:2000. Soil quality - Determination of nitrate nitrogen , ammonium nitrogen and total soluble nitrogen in air-dry soils using calcium chloride solution as extractant.

[11] TCVN 8940:2011. Soil quality - Determination of total phosphorus - Colorimetry method.

[12] TCVN 9716:2013. Water quality - General guidance on the enumeration of microorganisms by culture.

[13] Aminot, A., Kirkwood, D. S., and Kerouel, R., 1997. Determination of ammonia in seawater by the indophenol-blue method: Evaluation of the ICES NUTS I/C 5 questionnaire. Marine Chemistry, 56(1–2), 59–75. https://doi.org/10.1016/S0304-4203(96)00080-1.




DOI: https://doi.org/10.15625/1859-3097/15787 Display counter: Abstract : 21 views. PDF : 12 views.


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