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INVESTIGATION ON CONTENT OF FATTY ACIDS, PHOSPHOLIPIDS, AND PHOSPHOLIPID MOLECULAR SPECIES COMPOSITION OF THE VIETNAMESE CORAL Bebryce sp.

Dang Thi Phuong Ly, Pham Minh Quan, Trinh Thi Thu Huong, Valeria P. Grigorchuk, Pham Quoc Long, Luu Van Huyen, Andrey B. Imbs

Abstract


In the fatty acid composition of total lipid of the soft coral Bebryce sp., the concentration of unsaturated fatty acid predominates. The composition of saturated fatty acids is very diverse, including all saturated fatty acids from C14 to C26. The unsaturated fatty acids with high concentration are C20: 4n-6, 20:5n-3, 22:6n-3, 24:5n-6, 26:3n-6, 26:2n-6, 26:2n-7, 28:3n-6. In the fatty acids composition of the studied coral, there is presence of characterized fatty acids for the existence of sponges C25-C28 (demospongic fatty acids) with total content 29,86%. Most of the Bebryce coral species do not have zooxanthellae, therefore, in the fatty acids composition, either it is lack or contains only a small amount of markers fatty acids for zooxanthellae such as 18:5n-3, 18:4n-3, 18:3n-6, 20:4n-3. In the phospholipid content of the soft coral Bebryce sp., there is presence of characterized classes for Cnidarian animals such as phosphatidylethanolamine (PE), phosphatidylchonline (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphonolipid is ceramide aminoethylphosphonate (CAEP). PC account for the highest concentration (37,20% of total phospholipid). The molecular species of phospholipid classes of Bebryce sp. for the first time were investigated. In the results, there we 60 phospholipid molecular species identified in 5 classes. The molecular species with high content in the classes were PE 20:4/18:1e, PE 20:4/19:1, PC 20:4/18:0e, PC 20:4/16:0e, PS 24:5/18:0e, PI 24:5/18:0, CAEP18:2base/16:0 and CAEP 18:1base/16:0.

Keywords


Total lipid (TL), Polar lipid (PoL), molecular species, phospholipid, ethanolamine glycerophospholipid (PE), choline glycerophospholipid (PC), ceramide aminoethylphosphonate (CAEP), phosphatidylinositol (PI), serine glycerophospholipid (PS).

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References


Spener, F., Lagarde, M., Géloên, A., and Record, M., 2003. What is lipidomics?. European Journal of Lipid Science and

Technology, 105(9), 481-482.

Imbs, A. B., Dang, L. P., Rybin, V. G., Nguyen, N. T., and Pham, L.

Q., 2015. Distribution of very-long-chain fatty acids between molecular species of different phospholipid classes of two soft corals. Biochemistry and Analytical Biochemistry, 4(4), 205-206.

Imbs, A. B., Dang, L. P., Rybin, V. G., and Svetashev, V. I., 2015. Fatty acid, lipid class, and phospholipid molecular species composition of the soft coral Xenia sp. (Nha Trang bay, the South China Sea (Bien Dong Sea), Vietnam). Lipids, 50(6), 575-589.

Folch, J., Lees, M., and Sloane Stanley, G. H., 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226(1), 497-509.

Phattanawasin, P., Sotanaphun, U., Sriphong, L., Kanchanaphibool, I., and Piyapolrungroj, N., 2011. A comparison of image analysis software for quantitative TLC of ceftriaxone sodium. Science, Engineering and Health Studies (Former name “Silpakorn University Science and Technology Journal”), 5(1), 7-13.

Svetashev, V. T., and Vaskovsky, V. E., 1972. A simplified technique for thin-layer microchromatography of lipids. Journal of Chromatography

A, 67(2), 376-378.

Vaskovsky, V. E., and Terekhova, T. A., 1979. HPTLC of phospholipid mixtures containing phosphatidylglycerol. Journal of High Resolution Chromatography, 2(11), 671-672.

Kostetsky, E. Y., 1984. The phospholipid-composition of Spongia, Coelenterata, Plathelminthes, Nemertini, Annelida, Sipunculida and Echiurida. Biologiya Morya-Marine Biology, (5), 46-53.

Imbs, A. B., Demidkova, D. A., Dautova, T. N., and Latyshev, N. A., 2009. Fatty acid biomarkers of symbionts and unusual inhibition of tetracosapolyenoic acid biosynthesis in corals (Octocorallia).

Lipids, 44(4), 325-335.

Fabricius, K. K., and Alderslade, P. P., 2001. Soft corals and sea fans: a comprehensive guide to the tropical shallow water genera of the central-west Pacific, the Indian Ocean and the Red Sea. Australian Institute of Marine Science (AIMS).

Latyshev, N. A., Nguen, K. H., To, T. N., and Svetashev, V. I., 1986. Composition and seasonal fluctuations of alcyonarian phospholipids. Russ. J. Mar. Biol., 12, 178-182.

de Souza, L. M., Iacomini, M., Gorin, P. A., Sari, R. S., Haddad, M. A., and Sassaki, G. L., 2007. Glyco-and sphingophos-phonolipids from the medusa Phyllorhiza punctata: NMR and ESI-MS/MS fingerprints. Chemistry and Physics of Lipids, 145(2), 85-96.

Mukhamedova, K. S., and Glushenkova, A. I., 2000. Natural phosphonolipids. Chemistry of Natural Compounds, 36(4), 329-341.

Phạm Quốc Long, Imbs Andrey Borisovich, 2012. Lipit, axit béo và oxylipin của san hô. Nxb. Khoa học tự nhiên và Công nghệ. Tr. 125-184

Đặng Thị Phương Ly, 2016. Luận án Tiến sỹ: Nghiên cứu thành phần lipid và các dạng phân tử của phospholipid từ một số loài san hô mềm ở Việt Nam. Học viện Khoa học và công nghệ, Viện Hàn lâm Khoa học và Công nghệ Việt Nam.




DOI: https://doi.org/10.15625/1859-3097/18/2/12971

Journal of Marine Science and Technology ISSN: 1859 3097

Published by Vietnam Academy of Science and Technology