Synthesis of Gold Nanoparticles Conjugated with Protein A: Towards the Application in Biosensors for Virus Detection

Tran Quang Huy, Mai Anh Tuan

Abstract


In this article, a facile and effective technique is described to prepare a complex of gold nanoparticles (GNPs)/protein A (PrA) for biosensors in virus detection. GNPs were synthesized by the reduction of tetrachloroauric (III) acid trihydrate using sodium ascorbate, and then coated with PrA via ultracentrifugation. The complex of GNPs/PrA was characterized using UV-vis spectroscopy and transmission electron microscopy. The immunogold labeling method of scanning electron microscopy was also used to verify the capacity for the detection and binding of GNPs/PrA to H$_{1}$N$_{1}$ influenza A virus particles. The results showed that GNPs were spherical, uniform shape, and approximately 10 nm in size. Noticeably, the complex of GNPs/PrA could detect and bind effectively to H$_{1}$N$_{1}$ influenza A virus particles by a large number of GNPs surrounded. The advantage of the complex of GNPs/PrA showed a highly potential application in biosensors with the improvement of the sensitivity and transducing signal for virus detection.

Keywords


Gold nanoparticle, protein A, biosensor, immunosensor, virus detection

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DOI: https://doi.org/10.15625/0868-3166/21/4/359 Display counter: Abstract : 55 views. PDF : 29 views.

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